To indicate the threshold or level above which something will become unacceptable or will provoke a response; to create a boundary and imply or declare that its crossing will provoke a (negative) response.
To lay down a challenge; to provide a test of commitment (often one which carries a high risk) to a cause.
To create a real or artificial boundary or distinction between (two places, people or things).
To be negative; bring or cause negative results.
To perform the NOT operation on.
To deny the existence, evidence, or truth of; to contradict.
To nullify or cause to be ineffective.